|
| Status |
Public on May 21, 2020 |
| Title |
HMEC breast cell line |
| Sample type |
RNA |
| |
|
| Source name |
human commercial cell line, RNA extracted at 75% confluency
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HMEC
tumourigenic status: not tumourigenic
|
| Treatment protocol |
No treatment, cells were grown to 85% confluency prior to RNA extraction
|
| Growth protocol |
Cells were grown in culture using media as described in the repository from where each cell line was obtained. All cells were maintained at 37C in 5% CO2. All cells were either purchased within 4 years of use or validated to be the correct line using the Promega GenePrint 10 kit.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were washed with PBS and then Trizol-LS added directly to the cells, the manufacturers protocol was followed with the addition of glycogen during precipitation which was carried out overnight at -20C
|
| Label |
biotin
|
| Label protocol |
As per affymetrix protocol for preparation of biotinoylated cRNA using the FlashTag Biotin HSR RNA labelling kit. Input was 1μg of total RNA
|
| |
|
| Hybridization protocol |
As per affymetrix protocol
|
| Scan protocol |
As per affymetrix protocol using Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
Signals were normalized across all microarray chips using Robust Multi-array Analysis (RMA) designed and recommended by Affymetrix using GeneSpring GX software.
|
| |
|
| Submission date |
Mar 05, 2020 |
| Last update date |
May 21, 2020 |
| Contact name |
Judith Weidenhofer |
| E-mail(s) |
[email protected]
|
| Phone |
+612 49218706
|
| Organization name |
University of Newcastle
|
| Department |
School of Biomedical Sciences & Pharmacy
|
| Street address |
10 Chittaway Rd
|
| City |
Ourimbah |
| State/province |
NSW |
| ZIP/Postal code |
2258 |
| Country |
Australia |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE146477 |
Microarray analysis of miRNA in breast cancer cell lines |
|
