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Sample GSM4385159 Query DataSets for GSM4385159
Status Public on Mar 06, 2020
Title ceHBBoff1
Sample type SRA
 
Source name HEK 293 cells
Organism Homo sapiens
Characteristics tissue:  embryonic kidney
cell line: HEK 293
genotype: wild type
Extracted molecule genomic DNA
Extraction protocol Cells were collected after 48h post-transfection for genomic DNA extraction using FastPure Cell/Tissue DNA Isolation Mini Kit(Vazyme)
Libraries were prepared according to VAHTS Universal DNA Library Prep Kit for Illumina V2 (Vazyme)
 
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Illumina HiSeq 2000
 
Data processing The clean data was generated by trimming low quality bases at both ends of read and remove short fragments, this work was completed by sequencing company
For PAM depletion assay, we used custom Python or R scripts to get the result
For guide-seq, the sequencing data was processed by corresponding software named guideseq,the human genome version used in the analysis is hg19;
For deep-seq, the data was handled by CRISPRessoPooled
Genome_build: hg19
Supplementary_files_format_and_content: txt files include DNA sequences for each sample
 
Submission date Mar 05, 2020
Last update date Mar 06, 2020
Contact name chen peng
E-mail(s) [email protected]
Organization name Wuhan University
Department College of Life Sciences
Street address College of Life Sciences, Wuhan University
City Wuhan
ZIP/Postal code 430072
Country China
 
Platform ID GPL11154
Series (1)
GSE146420 A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing
Relations
BioSample SAMN14305424
SRA SRX7855619

Supplementary file Size Download File type/resource
GSM4385159_ceHBBoff1.txt.gz 606.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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