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Sample GSM435385 Query DataSets for GSM435385
Status Public on Jan 13, 2010
Title TEL-AML1-ALL, AIEOP data set (SI-TEL-15)
Sample type RNA
 
Source name White blood human cells
Organism Homo sapiens
Characteristics condition: TEL-AML1-ALL
genetic aberration: TEL-AML1
cell type: white blood cells
Treatment protocol White blood cells were isolated through Ficoll-Hypaque density gradient centrifugation
Growth protocol No growth protocol used
Extracted molecule total RNA
Extraction protocol Lysis of the mononuclear cells followed by lysate homonogenization using a biopolymer shredding system in a microcentrifuge spin-column format followed by total RNA purification using selective binding columns
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450DX.
Scan protocol GeneChips were scanned using the Scan GCS3000Dx.
Description Gene expression data from pediatric acute leukemia samples at diagnosis
Data processing Affymetrix Expression Console (EC) software Version 1.1 was used to median-normalize the data and then apply RMA.
 
Submission date Jul 31, 2009
Last update date Aug 28, 2018
Contact name Libi Hertzberg
Organization name Weizmann Institute of Science
Street address Herzel 145
City Rehovot
ZIP/Postal code 76100
Country Israel
 
Platform ID GPL570
Series (1)
GSE17459 Down's syndrome with acute lymphoblastic pediatric leukemia (DS-ALL)
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 2.35
AFFX-BioB-M_at 2.13
AFFX-BioB-3_at 3.03
AFFX-BioC-5_at 2.36
AFFX-BioC-3_at 2.36
AFFX-BioDn-5_at 1.95
AFFX-BioDn-3_at 5.82
AFFX-CreX-5_at 2.07
AFFX-CreX-3_at 2.16
AFFX-DapX-5_at 4.09
AFFX-DapX-M_at 4.91
AFFX-DapX-3_at 4.56
AFFX-LysX-5_at 2.34
AFFX-LysX-M_at 2.62
AFFX-LysX-3_at 3.6
AFFX-PheX-5_at 2.95
AFFX-PheX-M_at 3.15
AFFX-PheX-3_at 4.25
AFFX-ThrX-5_at 3.09
AFFX-ThrX-M_at 2.54

Total number of rows: 54675

Table truncated, full table size 838 Kbytes.




Supplementary file Size Download File type/resource
GSM435385_PAD_00466_U133.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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