|
| Status |
Public on Nov 19, 2009 |
| Title |
C58 3hpi 5 |
| Sample type |
RNA |
| |
|
| Source name |
wounded inflorescence stalk inoculated with C58
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Ws-2
tissue: wounded inflorescence stalk segments
inoculant: inoculated with Agrobacterium tumefaciens strain C58
|
| Treatment protocol |
The base of the first emerging main inflorescence stalk (3-5 cm) of intact plants was wounded with a syringe needle and inoculated with A. tumefaciens strain C58. Agrobacteria were scratched from the surface of an agar plate with a bent pipette tip by preventing contamination with medium and evenly spread on the wounded inflorescence stalk area immediately after wounding. Inoculated plants were grown for another three hours.
|
| Growth protocol |
Arabidopsis thaliana plants were cultivated in growth chambers under short-day conditions (8 h of light, 120 µE) at 22°C and 16°C during the dark period (16 h). Agrobacteria were grown on YEB plates over night.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted, including digestion of DNA, from plant tissue samples by using the RNeasy Plant Mini Kit according to manufacturer’s protocol (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Affymetrix protocol.
|
| Scan protocol |
The GeneChip® Scanner 3000 7G is also controlled by GeneChip® Operating Software(GCOS).
|
| Description |
Gene expression data of reference tissue were compared with those of inoculated tissue.
|
| Data processing |
VSN (variance stabilization normalization, reference: Huber et al. (2002) Bioinformatics 18: suppl. 1, S96-S104)
|
| |
|
| Submission date |
Jul 10, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Rosalia Deeken |
| E-mail(s) |
[email protected]
|
| Phone |
+49 931 8886121
|
| Fax |
+49 931 8886158
|
| Organization name |
University of Wuerzburg, Julius-von-Sachs-Institute
|
| Department |
Botany I; Plant Molecular Physiology & Biophysics
|
| Lab |
Prof. Hedrich
|
| Street address |
Julius-von-Sachs-Platz 2
|
| City |
Wuerzburg |
| ZIP/Postal code |
97072 |
| Country |
Germany |
| |
|
| Platform ID |
GPL198 |
| Series (2) |
| GSE13929 |
Arabidopsis thaliana three hours after infection with Agrobacterium tumefaciens |
| GSE14106 |
Transcriptome data from inflorescence stalks of intact A. thaliana plants after infection with A. tumefaciens |
|
| Relations |
| Reanalyzed by |
GSE118579 |
