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| Status |
Public on Nov 19, 2009 |
| Title |
Arabidopsis thaliana three hours after infection with Agrobacterium tumefaciens |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
This study focuses on responses of the host plant to infection with Agrobacterium tumefaciens. Genome wide changes in gene expression were integrated with the alterations in metabolite levels three hours after inoculation of agrobacteria. Plants were infected with the virulent strain C58, harboring a T-DNA, or with strain GV3101, containing a disarmed Ti-plasmid. This allows discrimination between signals which derive from the bacterial pathogen and the T-DNA encoded genes.
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| Overall design |
The bases of Arabidopsis thaliana (WS-2) inflorescence stalks were wounded and immediately infected with Agrobacterium tumefaciens or mock-infected for three hours. Stalks of intact plants were inoculated with the oncogenic strain C58 (C58 3 hpi 1 to 6) or the non-virulent strain GV3101 (GV3101 3 hpi 1 to 3) to provide conditions close to nature. The gene expression data of three independent experiments of infected material were compared with six non-infected samples (reference 3 hpi 1 to 6). Differential gene expression was determined by applying the LIMMA package (Linear Models for Microarray Data; Smyth, G.K. (2004) Applic. Genet. Mol. Biol. 3, Article 3; http://www.bepress.com/sagmb/vol3/iss1/art3/).
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| Contributor(s) |
Deeken R, Engelmann J |
| Citation(s) |
17172353, 19794116 |
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| Submission date |
Dec 12, 2008 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Rosalia Deeken |
| E-mail(s) |
[email protected]
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| Phone |
+49 931 8886121
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| Fax |
+49 931 8886158
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| Organization name |
University of Wuerzburg, Julius-von-Sachs-Institute
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| Department |
Botany I; Plant Molecular Physiology & Biophysics
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| Lab |
Prof. Hedrich
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| Street address |
Julius-von-Sachs-Platz 2
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| City |
Wuerzburg |
| ZIP/Postal code |
97072 |
| Country |
Germany |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE14106 |
Transcriptome data from inflorescence stalks of intact A. thaliana plants after infection with A. tumefaciens |
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| Relations |
| BioProject |
PRJNA114607 |
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