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| Status |
Public on Dec 01, 2021 |
| Title |
si-NC#1 |
| Sample type |
SRA |
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| Source name |
A2780
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| Organism |
Homo sapiens |
| Characteristics |
cell type: serous ovarian cancer cell line
transfection: si-NC
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
B2780si-Foxm1_case_vs_B2780si-NC_ctrl_all_list
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| Data processing |
Sequenced reads were generated by base calling using the Illumina standard pipeline
Raw reads were first filtered to obtain clean reads using FASTX-ToolkitÂ
High-quality reads were mapped to the human reference genome hg19 using TopHat software with default parameters
Alignment was independently performed for reads from each sample, and reads mapping to more than three genomic sites were discarded
Based on the value of reads per kilobase per million mapped reads, gene expression levels were determined using Cufflinks software
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| Submission date |
Dec 24, 2019 |
| Last update date |
Dec 01, 2021 |
| Contact name |
David Li |
| E-mail(s) |
[email protected]
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| Organization name |
Shandong University
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| Street address |
44 Wenhua Xi Road,Jinan
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| City |
jinan |
| ZIP/Postal code |
250012 |
| Country |
China |
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| Platform ID |
GPL24676 |
| Series (1) |
| GSE142567 |
RNA sequencing (RNA-SEQ) of FOXM1 knockdown by siRNA in ovarian cancer cells |
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| Relations |
| BioSample |
SAMN13676129 |
| SRA |
SRX7437707 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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