 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 01, 2021 |
| Title |
RNA sequencing (RNA-SEQ) of FOXM1 knockdown by siRNA in ovarian cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose：Forkhead box M1 (FOXM1) transcription factor is ubiquitously expressed in embryonic tissues but rare in differentiated cells. Increased expression of FOXM1 is observed in a variety of human malignancies. Here, we sought to characterize the expression and tumorigenic roles of FOXM1 in high-grade serous ovarian carcinoma (HGSOC). Experimental Design：TCGA dataset were analyed to identify potential master transcriptional regulators in HGSOC. Immunohistochemistry was performed to evaluate the clinical significance of FOXM1 in HGSOC. Invasion, clonogenic assays were conducted to determine to functional role of FOXM1. ChIP-seq and luciferase analyses were utilized to identify FOXM1 direct target genes in HGSOC. Association between mutant p53 and FOXM1 expression was investigated through TCGA data analysis, immunohistochemistry stainingand western blot. Results: We identified FOXM1 as a potential key transcription factor in ovarian cancer. FOXM1 was markedly increased in HGSOCs and high FOXM1 expression correlated with poor prognosis in HGSOC patients. Mechanistically, FOXM1 regulated CCNF and KIF20A at transcriptional level through binding on their promoters. Consistently, CCNF and KIF20A were elevated in HGSOCs and correlated with poor prognosis. Importantly, mutant p53 contribute to the high expression of FOXM1 in HGSOCs. Conclusions: These data reveal FOXM1 as a driver oncogenic transcription factor that promotes ovarian cancer malignancy which might be a potential drug target in HGSOC.
|
| |
|
| Overall design |
The assay was performed on three biological triplicates as well as three experimental triplictes of FOXM1 knockdown and control cells.
|
| |
|
| Contributor(s) |
Li Y, Liu Z, Kong B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 24, 2019 |
| Last update date |
Dec 01, 2021 |
| Contact name |
David Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Shandong University
|
| Street address |
44 Wenhua Xi Road,Jinan
|
| City |
jinan |
| ZIP/Postal code |
250012 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA597472 |
| SRA |
SRP238685 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142567_B2780si-Foxm1_case_vs_B2780si-NC_ctrl_all_list.xls.gz |
2.2 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...