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| Status |
Public on Sep 08, 2020 |
| Title |
T2KO_PGC (whs v17) |
| Sample type |
SRA |
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| Source name |
Primordial germ cell
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/129
age: E13.5
genotype: Tet2 knock out
cell type: Purified PGC from E13.5
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| Extracted molecule |
total RNA |
| Extraction protocol |
For oocyte sequence, we obtained 133 ovulated oocytes; for PGC sequence, we purified E13.5 PGC from 4-6 embryos each group
oocyte library were prepared using standard Illumina protocols; PGC library were prepared according to micro RNA construction
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
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| Description |
WT_PGC-VS-T2KO_PGC.PossionDis_Method.GeneDiffExp.txt
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| Data processing |
we analysed the raw data of fq.gz files by Hisat2,samtools and featurecounts to get the raw counts.
Genome_build: mm10
Supplementary_files_format_and_content: The reads counts of 133 samples, containg young WT, young Tet2-/-, old WT and old Tet2-/-, and some of Tet1 samples.
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| Submission date |
Dec 17, 2019 |
| Last update date |
Sep 08, 2020 |
| Contact name |
wang hua song |
| E-mail(s) |
[email protected]
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| Organization name |
nankai university
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| Street address |
Tianjin city, China
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| City |
Tianjin |
| ZIP/Postal code |
300071 |
| Country |
China |
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| Platform ID |
GPL23479 |
| Series (1) |
| GSE142163 |
Oocyte RNA-seq data of WT, Tet1-/- and Tet2-/-; RNA seq of PGC at E13.5 |
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| Relations |
| BioSample |
SAMN13612231 |
| SRA |
SRX7396412 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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