|
| Status |
Public on Jan 29, 2021 |
| Title |
GD18_M_C3_1 |
| Sample type |
RNA |
| |
|
| Source name |
GD18_male_control
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
age: fetal rat; gestational day 18
Sex: male
treatment: control
tissue: pituitaries
|
| Treatment protocol |
The tissues were immediately immersed in RNAlater stabilization reagent (QIAGEN).
|
| Growth protocol |
Pituitaries of male and female fetal Wistar rats at gestational day 18
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy kits (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
cRNA was amplified and labelled using a Low input Quick Amp Labelling Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
cRNA was hybridized to a 44K 60-mer oligomicroarray (Whole Rat Genome Microarray 4x44K v3; Agilent Technologies) according to the manufacturer's instructions.
|
| Scan protocol |
The hybridized microarray slides were scanned using an Agilent scanner.
|
| Description |
SAMPLE 3
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The raw signal intensities and flags for each probe were calculated from the hybridization intensities and spot information according to the procedures recommended by Agilent Technologies using the Flag criteria in the GeneSpring Software. The raw signal intensities of all samples were normalized by the quantile algorithm with the Bioconductor.
|
| |
|
| Submission date |
Oct 10, 2019 |
| Last update date |
Jan 29, 2021 |
| Contact name |
Tomoki Takeda |
| E-mail(s) |
[email protected]
|
| Organization name |
Japan Bioassay Research center
|
| Street address |
Hirasawa 2445
|
| City |
Hadano |
| ZIP/Postal code |
257-0015 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14746 |
| Series (1) |
| GSE138694 |
Effect of maternal dioxin exposure on pituitary mRNAs in fetal rats at gestational day 18 |
|
