NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM4065410 Query DataSets for GSM4065410
Status Public on Sep 07, 2019
Title BM_miRNA_0Gy_CBLB502_ip
Sample type RNA
 
Source name Bone Marrow, 12hr, 0 Gy, CBLB502 (i.p.)
Organism Mus musculus
Characteristics tissue: Bone marrow
gender: male
age: 6-8week
strain: C57BL/6
Treatment protocol The mice were pretreated with 10mg/kg Amifostime (i.p. 0.5 hr pre-IR), 0.2mg/kg CBLB502 (i.p. 0.5 hr pre-IR) and 150mg/kg Nilestriol (i.g. 48 hr pre-IR), and then radiated with 8 Gy gamma-radiation. At 12 hr post-radiation, samples of the bone marrow or hyprothalamus (pretreated with PBS or 10 mg/kg Amifostine) were obtained from the three mice per group.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted, pooled, and purified using miRNeasy Mini Kit (QIAGEN, GmBH, Germany) following the manufacturer’s instructions, and the RNA integrity number was verified on an Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA).
Label Cy3
Label protocol Total RNA was labeled by miRNA Complete Labeling and Hyb Kit (Cat#5190‐0456, Agilent technologies, Santa Clara, CA, US) followed the manufacturer’s instructions, labeling section.
 
Hybridization protocol Each slide was hybridized with 100ng Cy3‐labeled RNA using miRNA Complete Labeling and Hyb Kit (Cat# 5190-0456, Agilent technologies, Santa Clara, CA, US) in hybridization Oven (Cat#G2545A, Agilent technologies, Santa Clara, CA, US) at 55℃,20rpm for 20 hours according to the manufacturer’s instructions, hybridization section. After hybridization, slides were washed in staining dishes (Cat#121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat#5188‐5327, Agilent technologies, Santa Clara, CA, US).
Scan protocol Slides were scanned by Agilent Microarray Scanner (Cat#G2565BA, Agilent technologies, Santa Clara, CA, US) and Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US) with default settings.
Description miRNA expression after 12hr in 0 Gy-irradiated mice bone marrow pretreated with CBLB502 (i.p.)
Data processing Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
 
Submission date Sep 06, 2019
Last update date Sep 07, 2019
Contact name Changhui Ge
E-mail(s) [email protected], [email protected]
Organization name Beijing institute of Radiation Medicine
Department Department of Experimental Hematology and Biochemistry
Street address 27 Taiping Rd., Haidian Dist.
City Beijing
ZIP/Postal code 100850
Country China
 
Platform ID GPL17912
Series (2)
GSE137012 miRNA profiling in radiated mice bone marrow and hypothalamus pretreated with radioprotectants.
GSE137013 Profiling in radiated mice bone marrow and hypothalamus pretreated with radioprotectants

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
mmu-let-7a-1-3p -2.94
mmu-let-7a-2-3p -2.94
mmu-let-7a-5p 11.22
mmu-let-7b-3p 1.39
mmu-let-7b-5p 9.21
mmu-let-7c-1-3p -2.94
mmu-let-7c-5p 9.75
mmu-let-7d-3p -2.94
mmu-let-7d-5p 10.48
mmu-let-7e-3p -2.94
mmu-let-7e-5p 5.03
mmu-let-7f-1-3p -2.94
mmu-let-7f-2-3p -2.94
mmu-let-7f-5p 11.39
mmu-let-7g-3p -2.94
mmu-let-7g-5p 10.56
mmu-let-7i-3p -2.94
mmu-let-7i-5p 10.83
mmu-let-7j -2.94
mmu-let-7k -2.94

Total number of rows: 1247

Table truncated, full table size 25 Kbytes.




Supplementary file Size Download File type/resource
GSM4065410_BM_miRNA_0Gy_CBLB502_IP.txt.gz 7.9 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap