|
| Status |
Public on Jun 13, 2009 |
| Title |
mutated IGVH3-23 repl 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mutated purified cell of CLL
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Mutated purified cell of CLL
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted according to TRIZOL reagent protocol
|
| Label |
Cy5
|
| Label protocol |
Total RNA was labelled according to Ambion protocol. 825 ng of Cy5-labeled reference aRNA (according to Agilent protocol)
|
| |
|
| Channel 2 |
| Source name |
aRNA from pooled normal PB B cells of healthy donors
|
| Organism |
Homo sapiens |
| Characteristics |
reference: aRNA from pooled normal PB B cells of healthy donors
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted according to TRIZOL reagent protocol
|
| Label |
Cy3
|
| Label protocol |
Total RNA was labelled according to Ambion protocol. 825 ng of Cy3-labeled reference aRNA (according to Agilent protocol)
|
| |
|
| |
| Hybridization protocol |
The Hybridization was performed acording to Ambion protocol
|
| Scan protocol |
Slides were analysed by Agilent Microarray Scanner G2565AA
|
| Description |
Biological replicate 3 of 5.
|
| Data processing |
Background subtraction method: Marginal log-likelihood of foreground values for normal + exponential model and its derivatives.Whithin Normalization method: loess. Between Normalization method: quantile
|
| |
|
| Submission date |
May 12, 2009 |
| Last update date |
May 12, 2009 |
| Contact name |
daniela marconi |
| E-mail(s) |
[email protected]
|
| Organization name |
CRO AVIANO
|
| Street address |
Via franco Gallini 2
|
| City |
Aviano |
| ZIP/Postal code |
33081 |
| Country |
Italy |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
|
