|
| Status |
Public on Aug 06, 2019 |
| Title |
human iduced pluripotent stem cell, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
human induced pluripotent stem cells reprogrammed from the human foreskin fibroblast cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: human induced pluripotent stem cell
|
| Treatment protocol |
Cells were washed twice in PBS and then scraped and pelleted. Cell lysis was performed using miRNeasy mini kit (Qiagen) according to manufacturer's instructions.
|
| Growth protocol |
HFF (ATCC® CRL-2429™) were grown in IMDM medium (Biowest) supplemented with 10% Gibco™ Fetal Bovine Serum, Qualified, US Origin, Standard (Sterile-Filtered) (Gibco). IPSCs were grown in Nutristem (Biowest) on Laminin-521 (BioLamina) coated dish. Both cell lines mentioned below were cultured at 37◦C and 5% CO2 in a humidified Forma Steri-cycle i160 CO2 incubator (Thermo Fischer Scientific).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA isolation had been performed using the miRNeasy mini kit (Qiagen) according to the manufacturer’s protocol with DNaseI (Qiagen) treatment.
|
| Label |
biotin
|
| Label protocol |
Procedures for cDNA synthesis, target DNA fragmentation, and labelling were carried out according to the GeneChip WT PLUS Reagent Kit (Applied Biosystems) using 500 ng of total RNA as the starting material.
|
| |
|
| Hybridization protocol |
Hybridization on Affymetrix Gene Chip Human Transcriptome Array 2.0 microarrays, array washing, staining, and scanning were performed according to the manufacturer protocol.
|
| Scan protocol |
Scans of the microarrays were converted into CEL files using the scanner software, and were then processed using the Transcriptome Analysis Console 3.0.
|
| Description |
Gene expression data from human induced plutipotent stem cell
|
| Data processing |
Processed using the Transcriptome Analysis Console 3.0
|
| |
|
| Submission date |
Aug 05, 2019 |
| Last update date |
Aug 06, 2019 |
| Contact name |
Pierre Sabatier |
| E-mail(s) |
[email protected]
|
| Organization name |
Karolinska Institutet
|
| Department |
Medical Biochemistry and Biophysics
|
| Lab |
Chemistry I
|
| Street address |
Solnavägen 9
|
| City |
Solna |
| ZIP/Postal code |
171 65 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE135409 |
Expression data from human foreskin fibroblast and human iduced pluripotent stem cell |
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