|
| Status |
Public on Dec 10, 2009 |
| Title |
Cardiomyocytes_Pulsed-Blebbistatin_20090207 |
| Sample type |
RNA |
| |
|
| Source name |
Ventricular cardiomyocytes primary culture
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Lewis
developmental stage: 0-3 days neonatal
treatment: Electrical stimulated for 3 days at 1Hz, 2 Volt/cm;Supplemented with blebbistatin 10 µmole/L
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 11 ug of cRNA was hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix Gene Chip Scanner 7G
|
| Description |
Cardiomyocytes_Pulsed-Blebbistatin_20090207.CEL
|
| Data processing |
Data processed using PLIER in Affymetrix Expression Console V 1.1 with default settings, further processed using BRB-tools (http://linus.nci.nih.gov/BRB-ArrayTools.html): Normalized using housekeeping genes (rat orthologues of housekeeping genes on Affymetrix HG U133 plus 2 array)
|
| |
|
| Submission date |
Apr 27, 2009 |
| Last update date |
Oct 07, 2009 |
| Contact name |
Ruben Martherus |
| Organization name |
Maastricht University
|
| Department |
Clinical Genomics
|
| Street address |
Universiteitssingel 50
|
| City |
Maastricht |
| ZIP/Postal code |
6200 MD |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE15856 |
Electric stimulation of neonatal rat ventricular cardiomyocytes |
|
