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| Status |
Public on Dec 10, 2009 |
| Title |
Electric stimulation of neonatal rat ventricular cardiomyocytes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Study on changes in gene expression in primary cultures of neonatal rat ventricular cardiomyocytes to electric stimulation.
Through comparing non-stimulated, stimulated and blebbistatin supplemented and stimulated cultures we set out to identify the genes that are specifically activated by electric pulsing separate from cardiomyocyte contractions.
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| Overall design |
After initial recovery phase, primary cultures of neonatal rat ventricular cardiomyocytes were cultured for 3 days without pulsing, with electric pulsing or with electric pulsing combined with blebbistatin.
Per treatment: 3 arrays, with samples obtained from 3 separate series of cardiomyocyte isolation and culturing. Per array: sample prepared from pooled (1:1) RNA from duplicate experiments.
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| Contributor(s) |
Martherus RS, Vanherle SJ, Timmer ED, Zeijlemaker VA, Broers JL, Smeets HJ, Geraedts JP, Ayoubi TA |
| Citation(s) |
20858713 |
| |
| Submission date |
Apr 27, 2009 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Ruben Martherus |
| Organization name |
Maastricht University
|
| Department |
Clinical Genomics
|
| Street address |
Universiteitssingel 50
|
| City |
Maastricht |
| ZIP/Postal code |
6200 MD |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
|
| Samples (10)
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| GSM398199 |
Cardiomyocytes_Pulsed_20090131 |
| GSM398200 |
Cardiomyocytes_Pulsed-Blebbistatin_20090131 |
| GSM398201 |
Cardiomyocytes_Non-pulsed_20090207 |
| GSM398202 |
Cardiomyocytes_Pulsed_20090207 |
| GSM398203 |
Cardiomyocytes_Pulsed-Blebbistatin_20090207 |
| GSM398204 |
Cardiomyocytes_Pulsed_20090226 |
| GSM398205 |
Cardiomyocytes_Pulsed-Blebbistatin_20090226 |
|
| Relations |
| BioProject |
PRJNA116957 |
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