|
| Status |
Public on Aug 01, 2019 |
| Title |
2c_5 |
| Sample type |
RNA |
| |
|
| Source name |
2c hepatocytes
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain/background: Wistar
age: 6 weeks
Sex: Female
cell type: Hepatocytes
ploidy: 2c
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from sorted hepatocytes using the miRNeasy mini kit (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
Total RNA was amplified and labeled with Cyanine 3 (Cy3) using Agilent Low Input Quick Amp Labeling Kit, one-color (Agilent Technologies, Palo Alto, CA).
|
| |
|
| Hybridization protocol |
For each hybridization, 0.60 μg of Cy3 labeled cRNA were fragmented, and hybridized at 65 °C for 17 hours to an Agilent SurePrint G3 Rat GE v2 8x60K Microarray (Design ID: 074036).
|
| Scan protocol |
After washing, microarrays were scanned using an Agilent DNA microarray scanner.
|
| Description |
Gene expression in 2c hepatocytes.
|
| Data processing |
Intensity values of each scanned feature were quantified using Agilent Feature Extraction software version 10.7.3.1, which performs background subtractions.
Normalization was performed using Agilent GeneSpring version 12.6.1 (per chip: normalization to 75 percentile shift; per gene: normalization to median of all samples).
|
| |
|
| Submission date |
Jun 08, 2019 |
| Last update date |
Aug 01, 2019 |
| Contact name |
Takeshi Katsuda |
| E-mail(s) |
[email protected]
|
| Phone |
+1-215-746-5559
|
| Organization name |
University of Pennsylvania
|
| Department |
Perelman School of Medicine
|
| Lab |
Gastroenterology Division
|
| Street address |
421 Curie Blvd, BRB II/III Rm531
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL22740 |
| Series (1) |
| GSE132409 |
Transcriptome of adult rat hepatocytes with different ploidy statuses |
|
