tissue: Renal cortex strain: Crl:CD (SD) gender: male group: DKD+enarodustat
Extracted molecule
total RNA
Extraction protocol
Total RNA from kidney cortical tissue was isolated using GenElute Mammalian Total RNA Miniprep Kit (RTN70, Sigma-Aldrich). RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
0.6 ug of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes in a Agilent fragmentation buffer and was hybridized at 65°C for 17 hr using Gene Expression Hybridization Kit.
Scan protocol
Microarray slides were scanned at 3 μm resolution using an Agilent G4900DA SureScan Microarray Scanner System.
Description
Gene expression of renal cortex in DKD+enarodustat group
Data processing
The intensity data of each individual hybridization were extracted and the quality was assessed with the Feature Extraction Software 11.5.1.1(Agilent). Raw data were processed with the R package limma in Bioconductor to perform background correction and data normalization using the quantile normalization method, and batch effects were removed by ComBat (batch 1; SAMPLE 1, 2, 6, 7, 13, 14, batch 2; SAMPLE 3, 4, 8, 9, 15, 16, batch 3; SAMPLE 5, 10, 11, 12, 17, 18, 19).
