|
| Status |
Public on Dec 22, 2009 |
| Title |
HTCC1062_glucose30uM_latelog_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Bacteria, HTCC1062, late log phase
|
| Organism |
Candidatus Pelagibacter ubique HTCC1062 |
| Characteristics |
strain: HTCC1062
growth phase: late log
|
| Treatment protocol |
Cells were pelleted, treated with Bacteria Protect reagent (Qiagen) then frozen at -80C prior to extraction
|
| Growth protocol |
Cells grown 20C in dark, collected late log from seawater media via centrifugation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Rneasy kit (Qiagen) and amplified using MessageAmp-II-Bacteria RNA Amplification Kit (Ambion)
|
| Label |
biotin
|
| Label protocol |
RNA was labeled with biotin labeled nucleotides (perkin-elmer) during in vitro transcription (IVT) reaction.
|
| |
|
| Hybridization protocol |
5 ug of labeled aRNA was hybridized to chips at 45˚C, rotate at 60rpm overnight for 16 hours
|
| Scan protocol |
The samples were scanned with an Affymetrix GeneChip Scanner 3000 7G with autoloader using AGCC software.
|
| Description |
amplified aRNA, one IVT reaction with modified nucleotides
|
| Data processing |
RMA normalization conducted with Affymetrix ExpressionConsole software package, Statistical analyses performed in Multi-Experiment Viewer (MeV) software package (TIGR)
|
| |
|
| Submission date |
Feb 24, 2009 |
| Last update date |
Dec 22, 2009 |
| Contact name |
Michael S Schwalbach |
| Organization name |
University of Wisconsin-Madison
|
| Department |
Great Lakes Bioenergy Research Center
|
| Street address |
1550 Linden Dr
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL7330 |
| Series (1) |
| GSE14974 |
HTCC1062 glucose versus no carbon log phase |
|
