|
| Status |
Public on May 03, 2019 |
| Title |
P6-4-Nano-FFPE |
| Sample type |
RNA |
| |
|
| Source name |
Breast cancer tissue.
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast cancer
time point: later on-treatment biopsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from fresh frozen tissue using the Qiagen miRNeasy kit.
|
| Label |
N/A
|
| Label protocol |
N/A
|
| |
|
| Hybridization protocol |
300ng RNA was hybridised for 18hrs at 65C with custom NanoString capture probeset and detection codeset. Samples were held at 4C prior to purification and mobilisation at high sensitivity in the nCounter Prep Station (NanoString)
|
| Scan protocol |
555 fields of view (Max) were imaged per sample using the nCounter digital analyser (NanoString) and RCC (reporter code count) files exported for analysis
|
| Description |
Patient 6 later on-treatment biopsy sequenced using Nanostring technology from FFPE.
SAMPLE 3
|
| Data processing |
Using the nSolver 3.0 software
|
| |
|
| Submission date |
Apr 29, 2019 |
| Last update date |
May 04, 2019 |
| Contact name |
Carlos Martinez-Perez |
| E-mail(s) |
[email protected]
|
| Organization name |
The University of Edinburgh
|
| Department |
Institute of Genetics and Molecular Medicine
|
| Street address |
Western General Hospital, Crewe Road South
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL26593 |
| Series (2) |
| GSE130462 |
Unlocking the transcriptomic potential of formalin-fixed paraffin embedded clinical tissues: Comparison of gene expression profiling approaches [Nanostring] |
| GSE130645 |
Unlocking the transcriptomic potential of formalin-fixed paraffin embedded clinical tissues: Comparison of gene expression profiling approaches |
|
