|
| Status |
Public on Apr 20, 2019 |
| Title |
Hippocampus Aged Insulin Replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampus insulin 3 months
|
| Organism |
Rattus |
| Characteristics |
strain: F344
Sex: male
tissue: hippocampus
start age: 18 months
end age: 21 months
rin: 6.5
treatment: intranasal insulin for 3 months
|
| Treatment protocol |
All rats were treated with intranasal insulin aspart or saline (control) for 3 months, and were subjected to the Morris water maze task prior to hippocampus removal.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hippocampi were thawed on ice and homogenized in RiboZol™ Extraction Reagent (#97064-948; VWR®, Radnor, PA). RNA was precipitated with chloroform and isopropanol, then resuspended in a 75% ethanol solution.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA prepared according to standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16hr at 45 degrees C, on Clariom_S_Rat Affymetrix arrays (Affymetrix Fluidics Station 450)
|
| Scan protocol |
Scanning performed according to vendor instructions on Affymetrics GCS 3000 7G scanner. .CEL files produced were used for subsequent analyses.
|
| Description |
hippocampus from aged animal treated with intranasal insulin for 3 months
|
| Data processing |
.CEL files were processed with Partek software to RMA (log2) estimates of signal intensity.
|
| |
|
| Submission date |
Apr 19, 2019 |
| Last update date |
Apr 20, 2019 |
| Contact name |
Eric M Blalock |
| E-mail(s) |
[email protected]
|
| Phone |
859-323-8033
|
| Organization name |
University of Kentucky
|
| Department |
Molecular and Biomedical Pharmacology
|
| Lab |
Blalock
|
| Street address |
800 Rose St.
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40475 |
| Country |
USA |
| |
|
| Platform ID |
GPL23040 |
| Series (1) |
| GSE130098 |
Long-term intranasal insulin aspart: a profile of gene expression, memory, and insulin receptors in aged F344 rats |
|
