|
Status |
Public on Jun 27, 2019 |
Title |
Ctrl1_d22 |
Sample type |
SRA |
|
|
Source name |
VA10, a bronchial epithelial cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: VA10 cell type: bronchial epithelial cell line passages: 21 time point (days): 22
|
Treatment protocol |
Medium replaced 3x/week with addition of AZM or ethanol control.
|
Growth protocol |
Cells cultured in Transwell filter inserts with DMEM/F12 supplemented with 2%UltroserG
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA extracted with standard Trizol protocol. Libraries were prepared according to Illumina's instructions for HiSeq 2500
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Kallisto v0.43 Sleuth Genome_build: Ensembl 83 Supplementary_files_format_and_content: Kallist h5 or tsv abundance measurements
|
|
|
Submission date |
Mar 19, 2019 |
Last update date |
Jun 27, 2019 |
Contact name |
Zuzana Budkova |
Organization name |
University of Iceland
|
Department |
BioMedical Center, School of Health Sciences
|
Lab |
Stem Cell Research Unit
|
Street address |
Vatnsmyrarvegi 14
|
City |
Reykjavik |
ZIP/Postal code |
101 |
Country |
Iceland |
|
|
Platform ID |
GPL16791 |
Series (1) |
GSE128523 |
Azithromycin induces epidermal differentiation and multivesicular bodies in airway epithelia |
|
Relations |
BioSample |
SAMN11166116 |
SRA |
SRX5542809 |