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Sample GSM3678954 Query DataSets for GSM3678954
Status Public on Jun 27, 2019
Title Ctrl1_d22
Sample type SRA
 
Source name VA10, a bronchial epithelial cell line
Organism Homo sapiens
Characteristics cell line: VA10
cell type: bronchial epithelial cell line
passages: 21
time point (days): 22
Treatment protocol Medium replaced 3x/week with addition of AZM or ethanol control.
Growth protocol Cells cultured in Transwell filter inserts with DMEM/F12 supplemented with 2%UltroserG
Extracted molecule total RNA
Extraction protocol RNA extracted with standard Trizol protocol.
Libraries were prepared according to Illumina's instructions for HiSeq 2500
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing Kallisto v0.43
Sleuth
Genome_build: Ensembl 83
Supplementary_files_format_and_content: Kallist h5 or tsv abundance measurements
 
Submission date Mar 19, 2019
Last update date Jun 27, 2019
Contact name Zuzana Budkova
Organization name University of Iceland
Department BioMedical Center, School of Health Sciences
Lab Stem Cell Research Unit
Street address Vatnsmyrarvegi 14
City Reykjavik
ZIP/Postal code 101
Country Iceland
 
Platform ID GPL16791
Series (1)
GSE128523 Azithromycin induces epidermal differentiation and multivesicular bodies in airway epithelia
Relations
BioSample SAMN11166116
SRA SRX5542809

Supplementary file Size Download File type/resource
GSM3678954_Ctrl1_d22_kallisto_abundance.h5 79.8 Mb (ftp)(http) H5
GSM3678954_Ctrl1_d22_kallisto_abundance.tsv.gz 2.5 Mb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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