|
| Status |
Public on Aug 08, 2019 |
| Title |
RNAseq wt_2 [1223] |
| Sample type |
SRA |
| |
|
| Source name |
wiltype iPSC-derived neurons
|
| Organism |
Homo sapiens |
| Characteristics |
cell line background: 7889SA parental cell line
cell line source: 80 day old cortical neurons
cell type: co-isogenic iPS cells
mutation: none
|
| Growth protocol |
induction and maturation of cortical neurons from iPS cells (total of 80 days in vitro; detailed protocol in accompanying paper)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction, rRNA removal via Ribo-Zero treatment, DNAse-digestion
Illumina High-Throughput TruSeq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
alignment with STAR (default mode)
Genome_build: hg19
Supplementary_files_format_and_content: combined ReadsPerGene STAR output files (containing uniquely aligned reads per gene)
|
| |
|
| Submission date |
Mar 15, 2019 |
| Last update date |
Aug 08, 2019 |
| Contact name |
Claudia Scheckel |
| E-mail(s) |
[email protected]
|
| Organization name |
University Hospital Zurich
|
| Department |
Institute of Neuropathology
|
| Street address |
Schmelzbergstrasse 12
|
| City |
Zurich |
| State/province |
NY |
| ZIP/Postal code |
8091 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE128343 |
A large panel of isogenic APP and PSEN1 mutant human iPSC neurons reveals shared endosomal abnormalities mediated by APP b-CTFs, not Ab [RNA-seq] |
| GSE128345 |
A large panel of isogenic APP and PSEN1 mutant human iPSC neurons reveals shared endosomal abnormalities mediated by APP b-CTFs, not Ab |
|
| Relations |
| BioSample |
SAMN11130418 |
| SRA |
SRX5524958 |
