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| Status |
Public on Oct 11, 2019 |
| Title |
Day16_3 |
| Sample type |
SRA |
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| Source name |
REF cell line
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| Organism |
Rattus norvegicus |
| Characteristics |
cell type: cell with serum starvation for 16 days
transformation: none
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the cell pellets with RNA extraction kit (Zymo Research, R1050)
All the RNA products were confirmed with high quality (RQN score ≥ 7.5) using the Fragment AnalyzerTM platform (Advanced Analytical Technologies, Inc).
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Data processing |
Clean reads were aligned to rat reference rn19 using Tophat (version 2.1.1)
Genome_build: UCSC rn6
Supplementary_files_format_and_content: Cufflinks(cuffquant, cuffnorm version 2.2.1) were used to aquire normalized expression matrix (FPKM) covering all the samples.
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| Submission date |
Dec 19, 2018 |
| Last update date |
Oct 11, 2019 |
| Contact name |
ruoyan Li |
| E-mail(s) |
[email protected]
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| Organization name |
Peking University
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| Street address |
Yiheyuan street
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| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platform ID |
GPL18694 |
| Series (1) |
| GSE124109 |
Graded regulation of cellular quiescence depth between proliferation and senescence by a lysosomal dimmer switch |
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| Relations |
| BioSample |
SAMN10614918 |
| SRA |
SRX5164287 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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