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| Status |
Public on Oct 11, 2019 |
| Title |
Graded regulation of cellular quiescence depth between proliferation and senescence by a lysosomal dimmer switch |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The reactivation of quiescent cells to proliferate is fundamental to tissue repair and homeostasis in the body. Often referred to as the G0 state, quiescence is however not a uniform state but with graded depth. Shallow quiescent cells exhibit a higher tendency to revert to proliferation than deep quiescent cells, while deep quiescent cells are still fully reversible under physiological conditions, distinct from senescent cells. Cellular mechanisms underlying the control of quiescence depth and the connection between quiescence and senescence are poorly characterized, representing a missing link in our understanding of tissue homeostasis and regeneration. Here we measured transcriptome changes as rat embryonic fibroblasts moved from shallow to deep quiescence over time in the absence of growth signals. We found that lysosomal gene expression was significantly upregulated in deep quiescence, and partially compensated for gradually reduced autophagy flux. Reducing lysosomal function drove cells progressively deeper into quiescence and eventually into a senescence-like irreversibly arrested state; increasing lysosomal function, by lowering oxidative stress, progressively pushed cells into shallower quiescence. That is, lysosomal function modulates graded quiescence depth between proliferation and senescence as a dimmer switch. Lastly, we found that a gene expression signature developed by comparing deep and shallow quiescence in fibroblasts can correctly classify a wide array of senescent and aging cell types in vitro and in vivo, suggesting that while quiescence is generally considered to protect cells from irreversible arrest of senescence, quiescence deepening likely represents a common transition path from cell proliferation to senescence, related to aging.
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| Overall design |
Gene expression profiling of rat embryonic fibroblasts undergone different durations (0-16 days) of serum starvation, with 3 biological replicates at each time point.
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| Contributor(s) |
Bai F, Yao G |
| Citation(s) |
31636214 |
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| Submission date |
Dec 19, 2018 |
| Last update date |
Aug 08, 2024 |
| Contact name |
ruoyan Li |
| E-mail(s) |
[email protected]
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| Organization name |
Peking University
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| Street address |
Yiheyuan street
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| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA510786 |
| SRA |
SRP173916 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE124109_genes.processed.fpkm_table.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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