|
| Status |
Public on Mar 05, 2019 |
| Title |
Juvenile_mCherry_1 |
| Sample type |
SRA |
| |
|
| Source name |
Juvenile-mCherry
|
| Organism |
Drosophila melanogaster |
| Characteristics |
age: 0-3h post-eclosion
Sex: male
genotype/variation: UAS-unc84::GFP/+;R14H06-Gal4/UAS-mCherryRNAi
sample type: mushroom body nuclei
|
| Growth protocol |
Flies raised at 25°C and 70% humidity on standard agar growth medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mushroom body specific nuclei isolated using INTACT method (PMID:30158319), RNA extracted using Arcturus PicoPure RNA Isolation Kit (ThermoFisher: KIT0204)
RNA-Sequencing Library prepared using Nugen Ovation Universal RNA-Seq System for Drosophila (Nugen: NU035032)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Basecalls performed using RTA v2
RNA-Seq reads were trimmed using Prinseq-lite 0.20.4 quality aware trimming
Reads were aligned to the Drosophila genome using STAR 2.5.3a
Count tables were generated using HT-Seq Count v0.7.1 to count reads to genic features
Genome_build: BDGP release 6
Supplementary_files_format_and_content: processed data files are raw count tables in .txt format.
|
| |
|
| Submission date |
Nov 23, 2018 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Jamie M Kramer |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Western Ontario
|
| Department |
Physiology and Pharmacology
|
| Street address |
1151 Richmond St.
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A 3K7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE122864 |
Mushroom body-specific RNA sequencing to examine the role of Bap60, a core SWI/SNF subunit, in the regulation of neuronal genes. |
|
| Relations |
| BioSample |
SAMN10471752 |
| SRA |
SRX5057942 |
