|
| Status |
Public on Sep 01, 2021 |
| Title |
exp_3_adipocytes co-culture |
| Sample type |
RNA |
| |
|
| Source name |
WM3682 melanoma line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: WM3682
protocol: adipocytes co-culture
|
| Treatment protocol |
Exp.1 - WM1716 melnaoma cells were stably trasfected with scrambled control or miR-211. Exp.2 - WM3682 melnaoma cells were co-cultured with adipocytes or mono-cultured. RNA was extracted at day 5.
|
| Growth protocol |
Exp.1- WM1716 melanoma cells were grown in 10% serum DMEM. Exp.2- WM3682 melanoma cells were grown in adipocytes medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from WM1716 and WM3682 cells using Trizol (Qiagen), according to the manufacturer’s instructions. All samples were treated with TURBO DNA-free (Ambion) to remove trace amounts of genomic DNA.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with: miRNA-4_0 or HuGene-1_1-st-v1, as indicated (Affymetrix) and scanned at the HUJI Microarray Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RNA expression value derived from Affymetrix Launcher software
|
| Data processing |
Raw data were processed in R for background correction and RMA normalization. Normalized expression data were log-transformed.
|
| |
|
| Submission date |
Sep 05, 2018 |
| Last update date |
Sep 01, 2021 |
| Contact name |
Carmit Levy |
| E-mail(s) |
[email protected]
|
| Organization name |
Tel Aviv University
|
| Department |
Human Genetics and Biochemistry
|
| Lab |
Sackler School of Medicine
|
| Street address |
Ramat Aviv
|
| City |
Tel Aviv |
| ZIP/Postal code |
69978 |
| Country |
Israel |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE119478 |
Adipocytes induce melanoma plasticity by sensitizing cells to TGFb |
|
