|
| Status |
Public on Aug 27, 2018 |
| Title |
H545/15 T |
| Sample type |
RNA |
| |
|
| Source name |
Prostate biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Prostate biopsy
tissue pathology: Gleason 9 Cancer
treatment: treated with LTP
|
| Treatment protocol |
Cells were treated in a 12 well plate (100,000 cells/well) and exposed to Dielectric Barrier Discharge LTP jet at a distance of 15mm from cell media for 3 minutes.
|
| Growth protocol |
See Collins et al. (2005) Prospective identification of tumorigenic prostate cancer stem cells. Cancer Research. 2005 Dec 1;65(23):10946-51.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed in well using the Qiagen Rneasy animal cell extraction protocol, 2hrs after LTP treatment
|
| Label |
biotin
|
| Label protocol |
Labelling, hybridization and scanning were performed by Eurofins according to the Applied Biosystems GeneChip™ WT Pico Reagent Kit on the Clariom D Microarrays - See Manuals at; https://www.thermofisher.com/order/catalog/product/902622
|
| |
|
| Hybridization protocol |
Labelling, hybridization and scanning were performed by Eurofins according to the Applied Biosystems GeneChip™ WT Pico Reagent Kit on the Clariom D Microarrays - See Manuals at; https://www.thermofisher.com/order/catalog/product/902622
|
| Scan protocol |
Labelling, hybridization and scanning were performed by Eurofins according to the Applied Biosystems GeneChip™ WT Pico Reagent Kit on the Clariom D Microarrays - See Manuals at; https://www.thermofisher.com/order/catalog/product/902622
|
| Description |
Primary Gleason 9 prostate basal epithelial culture gene expression 2hrs post-3min LTP exposure
|
| Data processing |
Performed by JP on Affymetrix Transcriptome Analysis Console 3
|
| |
|
| Submission date |
Aug 26, 2018 |
| Last update date |
Aug 27, 2018 |
| Contact name |
John Packer |
| E-mail(s) |
[email protected]
|
| Organization name |
Mr
|
| Department |
Biology
|
| Lab |
Cancer Research Unit
|
| Street address |
Wentworth Way
|
| City |
York |
| State/province |
North Yorkshire |
| ZIP/Postal code |
YO10 5DD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE119052 |
Expression data from Low Temperature Plasma-treated primary prostate cultures |
|
