|
| Status |
Public on Aug 14, 2021 |
| Title |
158 microCurie_day14_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
158 microCurie_day14_rep2
|
| Organism |
Mus musculus |
| Characteristics |
tissue: whole blood
injected uci: 158
day: 14
|
| Treatment protocol |
Mice were injected with of 0, 158, 191, 215 and 259 microCurie of 137 CsCl solution.
|
| Growth protocol |
We obtained whole blood samples from injected mice (days 2, 3, 5, 7 and 14) from 6 replicates mice exposed to an internal emitter (cesium) in amounts of 0, 158, 191, 215 and 259 microCurie.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We isolated RNA from the blood using the PAXgene Blood RNA method (PreAnalytix GmBH, catalog#762165), and then depleted Globin transcripts using the Ambion GLOBINclear-Mouse kit (Life Technologies, Grand Island, NY, catalog#AM1980). We evaluated the samples for RNA integrity using the Agilent 2100 bioanalyzer and samples with RINs >8.0 were used for further analysis.
|
| Label |
Cy3
|
| Label protocol |
We processed 152 samples from each of the following groups: 0 (control), 158, 191, 215 and 259 uCi at days 2, 3, 5, 7 and day 14 RNA for microarray hybridization.
|
| |
|
| Hybridization protocol |
Agilent recommended protocols were used for hybridizations
|
| Scan protocol |
Slides were scanned with the Agilent DNA Microarray Scanner (G2505B), and the images were analyzed (Agilent Feature Extraction Software ver. 10.7) with default parameters for background correction and flagging non-uniform features.
|
| Description |
Gene expression in blood, 158 microCurie irradiated day 14
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Aug 16, 2018 |
| Last update date |
Aug 14, 2021 |
| Contact name |
Shanaz Adi Ghandhi |
| E-mail(s) |
[email protected]
|
| Phone |
212-3053911
|
| Organization name |
Columbia University Medical Center
|
| Department |
Center for Radiological Research
|
| Lab |
VC11-237
|
| Street address |
630, W 168th street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE118616 |
Mouse blood gene expression after administration of 137 Cesium |
|
