Mice were injected with of 0, 158, 191, 215 and 259 microCurie of 137 CsCl solution.
Growth protocol
We obtained whole blood samples from injected mice (days 2, 3, 5, 7 and 14) from 6 replicates mice exposed to an internal emitter (cesium) in amounts of 0, 158, 191, 215 and 259 microCurie.
Extracted molecule
total RNA
Extraction protocol
We isolated RNA from the blood using the PAXgene Blood RNA method (PreAnalytix GmBH, catalog#762165), and then depleted Globin transcripts using the Ambion GLOBINclear-Mouse kit (Life Technologies, Grand Island, NY, catalog#AM1980). We evaluated the samples for RNA integrity using the Agilent 2100 bioanalyzer and samples with RINs >8.0 were used for further analysis.
Label
Cy3
Label protocol
We processed 152 samples from each of the following groups: 0 (control), 158, 191, 215 and 259 uCi at days 2, 3, 5, 7 and day 14 RNA for microarray hybridization.
Hybridization protocol
Agilent recommended protocols were used for hybridizations
Scan protocol
Slides were scanned with the Agilent DNA Microarray Scanner (G2505B), and the images were analyzed (Agilent Feature Extraction Software ver. 10.7) with default parameters for background correction and flagging non-uniform features.
Description
Gene expression in blood, 158 microCurie irradiated day 7
Data processing
The scanned images were analyzed with Feature Extraction Software 10.7 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
