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Sample GSM3334358 Query DataSets for GSM3334358
Status Public on Jun 16, 2019
Title microRNA profile of NPC55
Sample type RNA
 
Source name 55
Organism Homo sapiens
Characteristics disease state: nasopharyngeal carcinoma
tissue: whole blood
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNA Purification Kit (Norgen Biotek, Thorold, Ontario, Canada).
Label pCp-DY647
Label protocol 2.5 μg of total RNA of nasopharyngeal carcinoma, head-neck tumours and normal controls whole blood was employed with 100 nmol/L of pCp-DY647 (Dharmacon, Lafayette, CO, USA) and 15 Units of T4 RNA ligase (USB, Cleveland, Oh, USA) in a total reaction volume of 20 µL at 16°C overnight.
 
Hybridization protocol The labeled RNAs were hybridized to the array with a 1× hybridization solution (5× Denhart’s solution, 0.5% SDS, 5× SSC) in a Hybridization Chamber (Corning Inc, Corning, NY, USA) at 46°C for 12 to 16 hours.
Scan protocol Scanned in a LuxScanTM -10K Scanner (capitalBio Inc., Beijing, China).
Data processing Quantile normalization across multiple arrays, log 2 transformation of expression levels, EXPression Analyzer and DisplayER (EXPANDER) version 4.1 was used.
 
Submission date Aug 15, 2018
Last update date Jun 16, 2019
Contact name Mei-Yin Zhang
E-mail(s) [email protected]
Organization name Sun Yat-sen University Cancer Center
Department State Key Laboratory of Oncology in South China
Street address 651 Dongfeng East Road
City Guangzhou
State/province Guangdong
ZIP/Postal code 510060
Country China
 
Platform ID GPL19888
Series (1)
GSE118613 Two miRNA signatures for identifying nasopharyngeal carcinoma from head-neck tumors and the normal

Data table header descriptions
ID_REF
VALUE Normalized log2 data represent microRNA expression levels

Data table
ID_REF VALUE
1 15.19318324
2 8.64385619
3 14.44221134
4 8.64385619
6 14.34143578
7 8.64385619
8 11.3419599
9 8.64385619
10 14.60697769
11 8.64385619
12 8.64385619
13 8.64385619
14 12.95474149
15 8.64385619
16 8.64385619
17 8.64385619
18 8.64385619
19 11.73650594
20 8.64385619
21 15.06713769

Total number of rows: 1856

Table truncated, full table size 28 Kbytes.




Supplementary file Size Download File type/resource
GSM3334358_20160728-s15-down-high100_NPC55.gpr.gz 139.1 Kb (ftp)(http) GPR
Processed data included within Sample table

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