|
| Status |
Public on Dec 31, 2009 |
| Title |
FAK transfected rat soleus muscle 425, unloaded + 5 days reloaded |
| Sample type |
RNA |
| |
|
| Source name |
Martin Flueck, Institute of Anatomy, University of Berne
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: soleus muscle
age: 3 month
gender: female
strain: Wistar
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Modified RNeasy mini kit (Qiagen)
|
| Label |
reverse transcription of 3 microgram total RNA with gene specific primers at 50degC using Superscript II RT (Invitrogen)
|
| Label protocol |
alpha-32P]dATP, and modified dCTP (Ambion (Europe)
|
| |
|
| Hybridization protocol |
ATLAS ARRAY-BD Clontech 16h at 68degC in ExpressHyb, Washing: 4x in (2xSSC, 1% SDS) at68degC for 1h. 1x in (0.1xSSC, 0.5% SDS) at68degC for ? h, 1x in (2x SSC) atroom temperature for 5min
|
| Scan protocol |
Phosphoimager screen, 4-10 days
|
| Description |
CMV-driven FAK expression construct was electro-transferred into a soleus muscle of an anesthetized rat. 2 days after transfection, the soleus muscles were unloaded for 7 days by hindlimb suspension. Subsequent reloading was provoked by allowing the animals to return to normal cage activity for 5 days. The muscle was isolated, snap frozen and total RNA extracted
|
| Data processing |
raw data
|
| |
|
| Submission date |
Sep 11, 2008 |
| Last update date |
Nov 03, 2010 |
| Contact name |
Martin Flück |
| E-mail(s) |
[email protected]
|
| Phone |
+41 31 631 4619
|
| Fax |
+41 31 631 3807
|
| Organization name |
University of Berne
|
| Department |
Department of Anatomy
|
| Lab |
Hoppeler
|
| Street address |
Bühlstrase 26
|
| City |
Berne 9 |
| State/province |
Bern |
| ZIP/Postal code |
3000 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL153 |
| Series (2) |
| GSE12747 |
pCMV-FAK vs pCMV in R5 |
| GSE16466 |
Transcriptome analysis of rat soleus muscle after altered loading state and somatic overexpression of FAK |
|
