|
| Status |
Public on Oct 29, 2018 |
| Title |
naive_3_A8957_1 |
| Sample type |
RNA |
| |
|
| Source name |
spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
Sex: female
strain: C57BL/6
parasite strain: naive
replicate mouse: 3
rna sample id: A8957
|
| Treatment protocol |
An inoculum containing 10×106 iRBC suspended in 100 ml of phosphate-buffered saline, pH 7.4, from donor mice was injected intravenously into each experimental C57BL/6 mouse to initiate infection. Naive mice receiving an equivalent number of uninfected RBCs served as a negative control group. Three to six mice as a group were infected with each parasite, and parasitemia were monitored by microscopic examination of Giemsa-stained thin tail blood smears.
|
| Growth protocol |
Inbred female C57BL/6 mice, aged 6–8 weeks, were purchased from Charles River Laboratory.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer or TapeStation.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol using HiScan-SQ
|
| Description |
spleen RNA 24 hr post infection with strain naive,mouse replicate 3, RNAsampleID=A8957
|
| Data processing |
Raw data filtered to include only probes detected in at least two arrays, using the detection_pvalue parameter output from Genome Studio, cutoff is 0.1; Each array normalized using quantile normalization of bead_average intensity, performed using JMP/Genomics software.
|
| |
|
| Submission date |
May 21, 2018 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Timothy G Myers |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institute of Allergy and Infectious Diseases
|
| Department |
Research Technologies Branch
|
| Lab |
Genomic Technologies Section
|
| Street address |
50 South Drive, Room 5509
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-8005 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE114718 |
Systematic detection of host pathways universally inhibited by Plasmodium yoelii parasites for immune intervention |
|
