|
| Status |
Public on Dec 31, 2008 |
| Title |
FoxO4 RNAi in C2C12 cells |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 cells
|
| Organism |
Mus musculus |
| Characteristics |
C2C12 cells were transfected with shRNA against FoxO4.
|
| Biomaterial provider |
Tjian lab
|
| Treatment protocol |
C2C12 cells were transfected with shRNA against FoxO4. Different colonies were screened for the best depletion of FoxO4.
|
| Growth protocol |
C2C12 cells were grown in DMEM with puromycine. All serums used were tested for cytotoxicity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit was used to extract total RNA.
|
| Label |
Biotin
|
| Label protocol |
Follow the protocol from Affymetrix.
|
| |
|
| Hybridization protocol |
Follow the protocol from Affymetrix.
|
| Scan protocol |
Follow the protocol from Affymetrix.
|
| Description |
C2C12 cells grown in DMEM transfected with FoxO4 shRNA.
|
| Data processing |
Using Affymetrix GeneChip GCOS expression analysis software to process.
|
| |
|
| Submission date |
Aug 15, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ping Hu |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California, Berkeley
|
| Department |
MCB
|
| Lab |
Tjian
|
| Street address |
Hearst & Oxford
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94564 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
|
| Relations |
| Reanalyzed by |
GSE119085 |
