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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 31, 2008 |
| Title |
FoxO RNAi in C2C12 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
C2C12 cells are mouse skeletal muscle cells. These cells were transfected with shRNA against FoxO1, FoxO3, and FoxO4. FoxO1, FoxO3, and FoxO4 are the known paralogues expressed in this cell line.
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| Overall design |
C2C12 cells are transfected with shRNA against FoxO1, FoxO3, and FoxO4, respectively. Colonies were selected for the best depletion of the target FoxOs, respectively. Cells were grown in DMEM medium. Total RNA was extracted from each line. Microarray analysis was performed by following the Affymetrix protocols. The data were analyzed by GCOS system. The target genes that we are interested in were further confirmed by qRT-PCR, reporter assay, and other biochemical and molecular biology assays.
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| Contributor(s) |
Hu P, Geles KG, Pairk J, DePinho RA, Tjian R |
| Citation(s) |
18854138 |
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| Submission date |
Oct 24, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Ping Hu |
| E-mail(s) |
[email protected]
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| Organization name |
University of California, Berkeley
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| Department |
MCB
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| Lab |
Tjian
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| Street address |
Hearst & Oxford
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| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94564 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA109825 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13347_RAW.tar |
10.6 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE13347_logratio.txt |
1.5 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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