|
| Status |
Public on Jul 29, 2008 |
| Title |
CD34+CD42- sorted cells from Unmobilized normal peripheral blood |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Reference probe: mixture of all RNAs tested in the experimental map
|
| Organism |
Homo sapiens |
| Characteristics |
Reference probe: mixture of all RNAs tested in the experimental map
|
| Biomaterial provider |
medical biomaterial
|
| Treatment protocol |
immunomagnetic positive selection of CD34+ cells
|
| Growth protocol |
no culture Day 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA trizol extraction
Quality control on bioanalyser 2100
Mix of all RNAs tested in the experimental map to realized reference probe before amplification and labeling protocol
|
| Label |
Cy3
|
| Label protocol |
direct labeling during in vitro transcription
|
| |
|
| Channel 2 |
| Source name |
pooled RNAs from 42 normal peripheral blood CD34+CD42b- sorted cells
|
| Organism |
Homo sapiens |
| Characteristics |
pooled RNAs from 42 normal peripheral blood CD34+CD42b- sorted cells
|
| Biomaterial provider |
medical biomaterial
|
| Treatment protocol |
immunomagnetic positive selection of CD34+ cells from normal peripheral blood (42 healthy donors). CD34+ CD42b- sorting on FACS DIVA (Becton Dickinson) to avoid platelet aggregation at surface of hematopoietic progenitors
|
| Growth protocol |
no culture Day 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA trizol extraction
Quality control on bioanalyser 2100
Mix of RNAs from 42 normal peripheral blood after sorting CD34+CD42b- cells
|
| Label |
Cy5
|
| Label protocol |
direct labeling during in vitro transcription
|
| |
|
| |
| Hybridization protocol |
dual hybridization on glass slide in Agilent hybridzation chamber.
|
| Scan protocol |
Agilent Scanner
Software Feature extraction from Agilent
Plateform Agilent of Gustave Roussy Institute (Villejuif, France)
|
| Description |
raw data requested but not available
|
| Data processing |
Extraction intensities, Background subtraction, normalization (LOWESS) by software Feature extraction (Agilent)
|
| |
|
| Submission date |
Jul 24, 2008 |
| Last update date |
Jul 28, 2008 |
| Contact name |
christophe desterke |
| E-mail(s) |
[email protected]
|
| Phone |
+33 (0)145595316
|
| Organization name |
university Paris Sud11
|
| Department |
UFR medecine institute Andre Lwoff
|
| Lab |
hopital Paul Brousse Batiment Lavoisier
|
| Street address |
14-16 avenue Paul Vaillant Couturier
|
| City |
VILLEJUIF |
| ZIP/Postal code |
94807 |
| Country |
France |
| |
|
| Platform ID |
GPL7091 |
| Series (1) |
| GSE12234 |
Transcriptome Study of CD34+ hematopoietic progenitor from patients reached of Primary Myelofibrosis |
|
