|
| Status |
Public on May 09, 2018 |
| Title |
Anthers - microspore stage, rep 1 |
| Sample type |
SRA |
| |
|
| Source name |
anther
|
| Organism |
Oryza sativa Japonica Group |
| Characteristics |
tissue: anther
Stage: microspore stage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Small RNA libraries were constructed using TruSeq Small RNA Sample Prep kits (Illumina, San Diego, CA). PARE libraries were constructed as previously described (Zhai et al., 2014). All libraries were sequenced on an Illumina HiSeq 2000 instrument at the Delaware Biotechnology Institute (Newark, DE).
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
small RNA
|
| Data processing |
Small RNA-seq: the adapter sequences were trimmed from raw sequence reads, and then the small RNA sequences were trimmed to the lengths between 18 and 34 nucleotides. Small RNA reads were mapped to the rice genome (MSU7).
PARE: the adapter sequences were trimmed from raw sequence reads, and then the small RNA sequences were trimmed to the lengths of 20 nucleotides. Small RNA reads were mapped to the rice genome (MSU7).
Genome_build: MSU7
|
| |
|
| Submission date |
Dec 18, 2017 |
| Last update date |
May 09, 2018 |
| Contact name |
Blake C. Meyers |
| E-mail(s) |
[email protected]
|
| Phone |
314-587-1422
|
| Organization name |
Donald Danforth Plant Science Center
|
| Lab |
Meyers lab
|
| Street address |
975 N Warson Road
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
| |
|
| Platform ID |
GPL13834 |
| Series (1) |
| GSE108238 |
Temporal expression of small RNAs during rice anther development |
|
| Relations |
| BioSample |
SAMN08200247 |
| SRA |
SRX3480951 |
