|
| Status |
Public on Mar 16, 2018 |
| Title |
S6_Pre |
| Sample type |
RNA |
| |
|
| Source name |
Human skeletal muscle, Vastus lateralis
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Vastus lateralis
gender: Male
subject: 6
time: Pre
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Biopsies were obtained from the vastus lateralis muscle using the Bergström needle technique. The biopsies were immediately stored in liquid nitrogen until processing. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HuGene-2_1-st array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Affymetrix protocol
|
| Description |
Subject 6, Pre
|
| Data processing |
The probe-set level intensities of the arrays were normalized using the Robust Multi-array Analysis method (RMA) implemented within Affymetrix Expression Console version 1.4.1.
|
| |
|
| Submission date |
Oct 16, 2017 |
| Last update date |
Mar 16, 2018 |
| Contact name |
Eric Rullman |
| E-mail(s) |
[email protected]
|
| Phone |
+46858586777
|
| Organization name |
Karolinska Institutet
|
| Department |
Laboratory Medicine
|
| Lab |
Clinical Physiology
|
| Street address |
C1:82 Karolinska University Hospital, Huddinge
|
| City |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17692 |
| Series (1) |
| GSE104999 |
Effects of 21 days of bedrest on human skeletal muscle gene expression |
|
