|
| Status |
Public on Aug 24, 2017 |
| Title |
ND or HFD -fed C57BL/6J mouse adipose tissues |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
ND-fed mouse adipose tissues
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J WT
tissue: Adipose tissue
|
| Treatment protocol |
Total RNA from mouse liver and adipose tissues were extracted using RNAiso Plus reagent following manufacturer's instructions. RNA quality was confirmed by Experion RNA gel (Biorad). RNA samples from 10 livers and 10 adipose tissues were combined in ND and HFD groups then used for a single microarray experiment
|
| Growth protocol |
C57BL/6J male mice were maintained under a 12-h light cycle at 21–25°C and a relative humidity of 50%–60% (SPF condition). Mice were fed a normal (ND) or a high-fat diet (HFD) for 4 weeks prior to tissue sampling.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10 µg of total RNA were primed with 2 µl of 100 µM T16N2 DNA primer at 70°C for 10 min, then reversed transcribed at 42°C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-label
|
| Label |
Cy3
|
| Label protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| |
|
| Channel 2 |
| Source name |
HFD-fed mouse adipose tissues
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J WT
tissue: Adipose tissue
|
| Treatment protocol |
Total RNA from mouse liver and adipose tissues were extracted using RNAiso Plus reagent following manufacturer's instructions. RNA quality was confirmed by Experion RNA gel (Biorad). RNA samples from 10 livers and 10 adipose tissues were combined in ND and HFD groups then used for a single microarray experiment
|
| Growth protocol |
C57BL/6J male mice were maintained under a 12-h light cycle at 21–25°C and a relative humidity of 50%–60% (SPF condition). Mice were fed a normal (ND) or a high-fat diet (HFD) for 4 weeks prior to tissue sampling.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10 µg of total RNA were primed with 2 µl of 100 µM T16N2 DNA primer at 70°C for 10 min, then reversed transcribed at 42°C for 1 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-label
|
| Label |
Cy5
|
| Label protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| |
|
| |
| Hybridization protocol |
Scanned on an Agilent G2565AA scanner.
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Aug 23, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Sung-Joon Joon Lee |
| E-mail(s) |
[email protected]
|
| Phone |
821089416868
|
| Organization name |
Korea University
|
| Department |
Biotechnology
|
| Lab |
Food Biomedical Science
|
| Street address |
145 Anam-Ro, Rm410, East Bldg., College of Life Sci&Biotechnol. Korea University
|
| City |
Seoul |
| ZIP/Postal code |
02841 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE102977 |
C57BL/6J WT mice liver and adipose: normal diet (ND) vs. high-fat diet (HFD) |
|
