|
| Status |
Public on Jun 01, 2020 |
| Title |
MX4 |
| Sample type |
RNA |
| |
|
| Source name |
Myocardium，IRI group
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Myocardium
gender: male
|
| Treatment protocol |
Rats in Jiaji group（JJ）, Neiguan group（NG）, Yanglingquan group(YL) and Quchi group(QC) were preconditon with 7-day treatment by acupuncturing apupoint respectively , MIRI model was replicated by ligation of the left anterior descending coronary artery in MX group and all acupuncture groups.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen) according to the manufacturer’s instructions. RNA integrity was assessed using standard denaturing agarose gel electrophoresis. RNA quantity and purity were determined by spectrophotometry (NanoDrop ND-1000 spectrophotometer)
|
| Label |
Cy3
|
| Label protocol |
Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology)
|
| |
|
| Hybridization protocol |
Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology)
|
| Scan protocol |
The hybridized arrays were washed, fixed, and scanned using the Agilent DNA Microarray Scanner (G2505B)
|
| Description |
Gene expression of IRI in rat myocardium
|
| Data processing |
Agilent Feature Extraction software (version 10.7.3.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed using the GeneSpring GX v11.5.1 software package (Agilent Technologies). After quantile normalization of the raw data, lncRNAs and mRNAs that at least 3 out of 15 samples had flagged as “Present” or “Marginal” were chosen for further data analysis. Differentially expressed lncRNAs and mRNAs were identified through fold change (FC) filtering. Differentially expressed lncRNAs and mRNAs with statistical significance (as determined by two-tailed student’s t-test < 0.05) were identified through Volcano Plot filtering. Hierarchical Clustering was performed using the Agilent GeneSpring GX software (version 11.5.1). *The Sample data table contains the quantile-normalized intensity from all probes that were detected in at least 3/15 samples prior to any data processing (e.g. prior to fold-change filtering). There was a considerable number of probes not detected in this experiment.
|
| |
|
| Submission date |
Jun 26, 2017 |
| Last update date |
Jun 01, 2020 |
| Contact name |
Qi Wen Tan |
| E-mail(s) |
[email protected]
|
| Phone |
+86 531 68616428
|
| Organization name |
Shandong University of TCM
|
| Department |
Acupuncture Department
|
| Lab |
Central Lab
|
| Street address |
Jingshi Road 16369
|
| City |
Jinan |
| State/province |
Shandong |
| ZIP/Postal code |
250014 |
| Country |
China |
| |
|
| Platform ID |
GPL14746 |
| Series (1) |
| GSE100499 |
Exploring acupuncture mechanism in preconditioning of IRI |
|
