|
| Status |
Public on Jan 30, 2008 |
| Title |
delta_ccpA_late_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
delta_ccpA, late growth phase in THY media
|
| Organism |
Streptococcus sp. 'group A' |
| Characteristics |
delta_ccpA is an isogenic mutant strain of MGAS5005
|
| Growth protocol |
Growth in standard laboratory media (THY) at 37 degrees with 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA protect was added at 2:1 volume. Cells were harvested via centrifugation and snap frozen. Pellets were resuspended and nucleic acid harvested using phenol-chloroform extraction. RNA was isolated using Qiagen RNeasy mini-kits.
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix Protocol via Affymetrix labeling kit.
|
| |
|
| Hybridization protocol |
Standard Affymetrix for low G+C bacteria.
|
| Scan protocol |
Standard Affymetrix.
|
| Description |
Care was taken during sample processing to ensure equivalent amounts of cDNA were hybridized to each chip.
|
| Data processing |
Values were generated using GCOS.
|
| |
|
| Submission date |
Jan 11, 2008 |
| Last update date |
Jan 30, 2008 |
| Contact name |
Samuel Shelburne |
| E-mail(s) |
[email protected]
|
| Phone |
713-792-3629
|
| Organization name |
MD Anderson Cancer Center
|
| Department |
Infectious Diseases
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6186 |
| Series (1) |
| GSE10156 |
Analysis of the group A Streptococcus CcpA Transcriptome |
|
