genotype/variation: wild type parent phenotype: normal
Treatment protocol
Arabidopsis 6 weeks old plants were infected by rapid immersion in a bacterial suspension (5. 107 cfu/mL)
Growth protocol
Bacteria were isolated from Arabidopsis leaf surfaces (6 hpi) or from whole leaf (24 hpi) using the protocol described in Chapelle, E., Alunni, B., Malfatti, P., Solier, L., Pédron, J, Kraepiel, Y., and Van Gijsegem, F. (2015) . A straightforward and reliable method for bacterial in planta transcriptomics: application to the Dickeya dadantii/Arabidopsis thaliana pathosystem. Plant J 82: 352-362.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted by the hot phenol method and DNA was removed by AMBION Dnase treatment
Label
Cy3
Label protocol
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
Hybridization protocol
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
Scan protocol
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
Description
Wild type bacteria isolated from arabidopsis leaves 6 hours post inoculation for the comparison with Wild type bacteria isolated from arabidopsis leaves 24 hours post inoculation replicat 2
Data processing
The raw data (.pair file) were analyzed with the ANAIS web interface (http://anais.versailles.inra.fr/; Simon, A., and Biot, E. (2010). ANAIS: Analysis of NimbleGen Arrays Interface. Bioinformatics 26: 2468-2469).
