|
| Status |
Public on Dec 01, 2017 |
| Title |
3130F 7_19 |
| Sample type |
RNA |
| |
|
| Source name |
melanomaF
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: melanoma cells cultured in stem cell medium
|
| Growth protocol |
Human melanocytes were grown in 254CF medium before they reach the 70% confluency. Melanoma cells in MelanomaA subgroup were grown in Tumor 2% medium before they reach the 70% confluency. Melanoma cells in MelanomaF subgroup were grown in StemPro medium. NCSC were maintained in StemPro medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were washed again with HBSS and lysed directly in TRI Reagent® RNA Isolation Reagent (Sigma) and stored at -20°C.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
s74
Melanoma cells (WM3130_F)
|
| Data processing |
The data were normalised using quantile normalisation with lumi in R
|
| |
|
| Submission date |
Dec 22, 2016 |
| Last update date |
Dec 01, 2017 |
| Contact name |
Jianglan Liu |
| E-mail(s) |
[email protected]
|
| Organization name |
The Wistar Institute
|
| Street address |
3601 Spruce Street
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL8234 |
| Series (2) |
| GSE92763 |
LPAR signaling activated in neural crest stem cells promotes melanoma cell growth, invasion and therapy resistance [HumanWG6_V2] |
| GSE92765 |
LPAR signaling activated in neural crest stem cells promotes melanoma cell growth, invasion and therapy resistance |
|
