|
| Status |
Public on Oct 03, 2017 |
| Title |
CS22747 Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Aerial parts of seedling
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
accession: CS22747
age: 18-days-old
tissue: Aerial parts of seedling
|
| Growth protocol |
18-days-old seedlings were growing on 1% agar plates containing Murashige and Skoog (MS)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 500 μg total RNA was extracted from each sample using TRIzol (Invitrogen)
|
| Label |
Cy3
|
| Label protocol |
labeling were performed using the Agilent Low RNA Input Linear Amplification Kit (Agilent Technology), according to the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Hybridization was performed using the in situ Hybridization Kit Plus (Agilent Technologies).
|
| Scan protocol |
Scanned on an Agilent G2505 BDNA microarray scanner (Agilent Technologies)
|
| Description |
Gene expression of CS22747
|
| Data processing |
Data analysis was performed by Feature Extraction and Image Analysis software (Agilent Technologies)
|
| |
|
| Submission date |
Nov 14, 2016 |
| Last update date |
Oct 03, 2017 |
| Contact name |
Kazumasa Shirai |
| E-mail(s) |
[email protected]
|
| Phone |
0948-29-7842
|
| Organization name |
Kyushu Institute of Technology
|
| Department |
Frontier Research Academy for Young Researchers
|
| Lab |
Hanada lab
|
| Street address |
680-4 Kawazu
|
| City |
Iizuka |
| State/province |
Fukuoka |
| ZIP/Postal code |
820-8502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14937 |
| Series (1) |
| GSE89805 |
Transcriptome analysis of annotated coding genes and sORF at 74 accessions of A. thaliana |
|
