|
| Status |
Public on Oct 25, 2016 |
| Title |
3 day treatment [Mendias_003_03] |
| Sample type |
RNA |
| |
|
| Source name |
plantaris muscle, synergist ablation, PDGF inhibitor used
|
| Organism |
Mus musculus |
| Characteristics |
time point: 3 day
group: treatment
strain: C57BL/6
Sex: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiazol RNA extraction
|
| Label |
biotin
|
| Label protocol |
cDNA were prepared according to the Nugen Ovation PicoSL WTA System V2 kit protocol from 10 ng total RNA (Ovation PicoSL WTA System V2 Manual, P/N M01226 v4). Subsequently, Biotinylated cDNA were prepared according to the Nugen Encore Biotin Module from 3.75 ug of cDNA (Encore Biotin Module Manual, P/N M01111 v6)
|
| |
|
| Hybridization protocol |
Following Labeling procedure, 3.75 ug of biotinylated cDNA were hybridized at 48C – WT on Human Gene ST 2.1 strip arrays, and were washed and stained using the Affymetrix Gene Atlas system (software version 1.0.4.267)
|
| Scan protocol |
Arrays were scanned using the Affymetrix Gene Atlas system (software version 1.0.4.267)
|
| Description |
prepared using the GeneChip WT PLUS Reagent Kit and hybridized to Mouse Gene 2.1 ST Array Strips
|
| Data processing |
Data was analyzed using the limma and oligo Bioconductor packages implemented in the R-statistical environment version 3.3.0. RMA was used to fit log2 transformed expression values
|
| |
|
| Submission date |
Oct 24, 2016 |
| Last update date |
Oct 25, 2016 |
| Contact name |
Christopher Louis Mendias |
| E-mail(s) |
[email protected]
|
| Phone |
734-764-4206
|
| Organization name |
University of Michigan
|
| Department |
Orthopaedic Surgery
|
| Lab |
Mendias Lab
|
| Street address |
109 Zina Pitcher Place
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE89111 |
Targeted inhibition of platelet-derived growth factor signaling prevents muscle fiber growth during skeletal muscle hypertrophy |
|
