|
Status |
Public on Sep 30, 2016 |
Title |
Control - 3 |
Sample type |
RNA |
|
|
Source name |
Ago2-Amygdala IP without social defeat
|
Organism |
Mus musculus |
Characteristics |
tissue: Mouse brain amygdala strain: C57BL/6J
|
Treatment protocol |
Amygdala samples were collected from social defeat and control mice 8 days after the end of the chronic social defeat protocol
|
Growth protocol |
10-week old C57BL/6J male mice were subjected to a chronic social defeat protocol
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the RNeasy kit, miRNA supplementary protocol (QIAGEN, Hilden, Germany).
|
Label |
biotin
|
Label protocol |
RNA was labeled using Flashtag RNA labeling kit
|
|
|
Hybridization protocol |
Samples were hybridized to a GeneChip miRNA 2.0 Array (Affymetrix)
|
Scan protocol |
Chips were scanned on a GeneChip Scanner 3000 7G (Affymetrix)
|
Description |
total RNA extracted from the Ago2-amygdala complex following an Ago2 IP miRNA expression data from mice Ago2-amygdala without social defeat
|
Data processing |
The data was analyzed with Affymetrix miRNA QC tool and normalized using default parameters
|
|
|
Submission date |
Sep 29, 2016 |
Last update date |
Sep 30, 2016 |
Contact name |
Alon Chen |
Organization name |
The Weizmann Institute of Science
|
Department |
Neurobiology
|
Street address |
234 Herzel St.
|
City |
Rehovot |
ZIP/Postal code |
7610001 |
Country |
Israel |
|
|
Platform ID |
GPL14613 |
Series (2) |
GSE87488 |
miRNA expression data from mice Ago2-amygdala |
GSE87490 |
Amygdalar microRNA-15a is essential for coping with chronic stress |
|