|
| Status |
Public on Sep 22, 2016 |
| Title |
Male HP_sham_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Male hippocampus_sham
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
gender: male
experimental procedure: sham-operated
tissue: brain
brain region: Hippocampus
|
| Treatment protocol |
Wistar rats were housed under a 12-h light, 12-h dark cycle, with food and water ad libitum during the course of the experiment. Male rats (2 months) were orchidectomized or sham-operated. Two weeks after surgery, castrated and sham animals were anesthetized with a mixture of ketamine and medetomidine. Cerebral hemispheres were separated and HP collected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following manufacturer’s instructions. RNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies), and RNA integrity was assessed by Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). All RNA samples used in this study had an A260/A280 ratio of.1.9
|
| Label |
Cy3
|
| Label protocol |
Equal amounts of RNA extract (200 ng) from the HP of sham or OOX animals, in a total of three biological replicates, were amplified and Cy- 3-labeled using the Low Input Quick Amp Labeling kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Hybridizations were carried out on an Agilent-based microarray platform using custom-designed whole genome Rat GE 4x4K v3 microarrays
|
| Scan protocol |
Scanned images on an Agilent microarray scanner were analyzed by Feature extraction software (Agilent Technologies) using GE1_105_Dec08 protocol.
|
| Description |
HP gene expression in sham males
|
| Data processing |
The signal intensity was normalized by centering the median of the signal distribution using BRB-ArrayTools v3.8.1 (http://linus.nci.nih.gov/BRB-ArrayTools.html).
|
| |
|
| Submission date |
Sep 21, 2016 |
| Last update date |
Sep 22, 2016 |
| Contact name |
Telma Quintela |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Beira Interior
|
| Department |
Health Sciences Research Centre
|
| Street address |
Av. Infante D. Henrique
|
| City |
Covilhã |
| ZIP/Postal code |
6200-506 |
| Country |
Portugal |
| |
|
| Platform ID |
GPL14746 |
| Series (1) |
| GSE87188 |
Gene Expression Profiling in the Hippocampus of Orchidectomized Rats |
|
