|
| Status |
Public on Jul 31, 2017 |
| Title |
glomeruli from JAK2 Akita diabetic glomeruli + JAK1/2 inhibitor 5 |
| Sample type |
RNA |
| |
|
| Source name |
glomeruli from diabetic JAK2 mouse treated with JAK1/2 inhibitor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Glomeruli
disease state: diabetic
treatment: JAK1/2 inhibitor
|
| Treatment protocol |
129S6 Akita podocyte-JAK2 transgenic mice were implanted with osmotic minipumps that infused angiontensin II subcutaneously at a rate of 700 ng/min/kg for 4 wks. During the final 2 wks of this infusion the experimental mice received a JAK1/2 inhibitor at 3 mg/kg/day in acidified water, while the control mice received vehicle alone. The JAK1/2 inhibitor reduced mesangial expansion and albuminuria in the diabetic hypertensive mice without affecting glycemia or blood pressure.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy Mini Kit (Qiagen, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix Standard protocol
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene expression data from JAK2 mice (JAK1/2 ) glomeruli (16 weeks old)
|
| Data processing |
The data were analyzed using ChipInspector which carries out significance analysis on the single probe level. Normalized probe set level data not provided for individual Sample records. ChipInspector gives the log2 based fold change ratio between two group samples for each gene
|
| |
|
| Submission date |
Aug 31, 2016 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Viji Nair |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Michigan
|
| Street address |
1152 West Medical Centre Dr.
|
| City |
Ann Arbor |
| ZIP/Postal code |
48331 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE86317 |
Podocyte-specific JAK2 Overexpression Worsens Diabetic Kidney Disease in Mice |
|
