|
| Status |
Public on May 07, 2008 |
| Title |
Cerevisiae_CyclinMutant_158min_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Cerevisiae culture, cyclin mutant, 158 min
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain: BF264-15DU MATa; clb1::URA3; clb2::LEU2; clb3::TRP1; clb4::HIS2; clb5::ARG4; clb6::APE1; GAL1-CLB1:LEU2
|
| Treatment protocol |
synchronous G1 populations collected by elutration.
|
| Growth protocol |
YEPDextrose /1M Sorbitol at 30c
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using standard yeast methods.
|
| Label |
biotin
|
| Label protocol |
Amplification and labeling using the GeneChip One-Cycle target Labeling (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
DNA microarray hybridization was performed according to standard Affymetrix protocols by the Duke Micorarray Core Facility.
|
| Scan protocol |
Array scanning was carried by the Duke Micorarray Core Facility using standard Affymetrix protocols.
|
| Description |
Cell cycle gene expression data from B-cyclin mutant yeast synchronized by elutration
|
| Data processing |
Data from all 60 chips was normalized together using the dChip implementation with default paramaters from the affy package (v1.8.1) within Bioconductor in the R statistical environment.
|
| |
|
| Submission date |
Aug 16, 2007 |
| Last update date |
Mar 24, 2017 |
| Contact name |
Steven B Haase |
| URL |
http://www.biology.duke.edu/haaselab/
|
| Organization name |
Duke University
|
| Department |
Biology
|
| Street address |
Duke University, Department of Biology, Box 90338
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708-1000 |
| Country |
USA |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE8799 |
Global Control of cell cycle transcription by coupled CDK and network oscillators |
|
| Relations |
| Reanalyzed by |
GSE75694 |
| Reanalyzed by |
GSM2549595 |
