|
| Status |
Public on Sep 22, 2017 |
| Title |
Cerevisiae_CyclinMutant_158min_rep2_reanalyzed |
| Sample type |
RNA |
| |
|
| Source name |
S. cerevisiae culture CyclinMutant 158min
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BF264-15DU MATa; clb1::URA3; clb2::LEU2; clb3::TRP1; clb4::HIS2; clb5::ARG4; clb6::APE1; GAL1-CLB1:LEU2
|
| Treatment protocol |
synchronous G1 populations collected by elutration.
|
| Growth protocol |
YEPDextrose /1M Sorbitol at 30c
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using standard yeast methods.
|
| Label |
biotin
|
| Label protocol |
biotin
|
| |
|
| Hybridization protocol |
DNA microarray hybridization was performed according to standard Affymetrix protocols by the Duke Micorarray Core Facility.
|
| Scan protocol |
Array scanning was carried by the Duke Micorarray Core Facility using standard Affymetrix protocols.
|
| Description |
Cell cycle gene expression data from B-cyclin mutant yeast synchronized by elutration
|
| Data processing |
The new samples in this study and re-analyzed samples from GSE8799, GSE32974, and GSE49650 were normalized together using the dChip implementation with default parameters from the affy package within R/Bioconductor. S. pombe probes are removed before analysis.
|
| |
|
| Submission date |
Mar 24, 2017 |
| Last update date |
Sep 23, 2017 |
| Contact name |
Steven B Haase |
| E-mail(s) |
[email protected]
|
| Organization name |
Duke University
|
| Department |
Biology
|
| Street address |
Box 91000
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE96997 |
Investigating global transcript dynamics in mitotically arrested budding yeast cells |
|
| Relations |
| Reanalysis of |
GSM218650 |
