|
| Status |
Public on Jan 01, 2017 |
| Title |
wt SAL 5 |
| Sample type |
RNA |
| |
|
| Source name |
Spinal cord of 6-10 week old C57BL/6J mice
|
| Organism |
Mus musculus |
| Characteristics |
chronic treatment: WT
acute treatment: SAL
genotype/variation: C57BL/6J
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled using the Affymetrix/Ambion WT Expression kit, combined with Affymetrix GeneChip WT terminal labeling kit.
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol (MOUSE GENE 2.1 atlas microarrays).
|
| Scan protocol |
Standard Affymetrix scanning protocol.
|
| Data processing |
The data were processed using oligo R package normalised using quantile normalisation.
|
| |
|
| Submission date |
Apr 21, 2016 |
| Last update date |
Jan 01, 2017 |
| Contact name |
Marcin Piechota |
| E-mail(s) |
[email protected]
|
| Phone |
(+4812) 6623328
|
| Organization name |
Institute of Pharmacology PAS
|
| Department |
Molecular Neuropharmacology
|
| Street address |
Smetna 12
|
| City |
Krakow |
| ZIP/Postal code |
31-343 |
| Country |
Poland |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE80504 |
Astrocyte-specific transcriptional response to glucocorticoid receptor activation in the central nervous system |
|
