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| Status |
Public on Jan 01, 2017 |
| Title |
Astrocyte-specific transcriptional response to glucocorticoid receptor activation in the central nervous system |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Many studies indicated in an indirect way that astrocytes are a major target for transcriptional responses induced by glucocorticoids in the CNS, implying that glial cells an important consituent of stress responses. To address this issue directly we have generated astrocyte-specific conditional GR knockout mice (Cx30GRko) using Cre/loxP system utilizing connexin 30 promoter driving expression of CreERT2 recombinase.
Genome wide analysis of molecular effects of astrocytic GR knockout would allow for dissecting the glial cell-type specific components of stress response mediated by glucocorticoids
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| Overall design |
We have analyzed 4 groups (5 mice/group): control animals injected with saline, Cx30GRko animals injected with saline, control animals injected with GR agonist dexamethasone (Dex, 4 mg/kg, i.p.) and Cx30GRko animals injected with Dex (4 mg/kg, i.p.). Spinal cord (tissue characterized by the highest efficiency of Cre-mediated recombination) samples were collected 4h after injections.
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| Contributor(s) |
Slezak M, Tertil M, Korostynski M, Piechota M, Przewlocki R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 21, 2016 |
| Last update date |
Mar 08, 2018 |
| Contact name |
Marcin Piechota |
| E-mail(s) |
[email protected]
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| Phone |
(+4812) 6623328
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| Organization name |
Institute of Pharmacology PAS
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| Department |
Molecular Neuropharmacology
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| Street address |
Smetna 12
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| City |
Krakow |
| ZIP/Postal code |
31-343 |
| Country |
Poland |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA319150 |
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